Results
| PMID | 21640344 |
| Gene Name | INHA |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | Activin-A |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2011 Jul;96(1):118-21. doi: 10.1016/j.fertnstert.2011.05.006. Epub Yoshino, Osamu| Izumi, Gentaro| Shi, Jia| Osuga, Yutaka| Hirota, Yasushi| Hirata, Tetsuya| Harada, Miyuki| Nishii, Osamu| Koga, Kaori| Taketani, Yuji Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan. oyoshino624@hotmail.co.jp OBJECTIVE: To examine the regulation and the function of activin-A in stromal cells derived from endometrioma. DESIGN: Molecular studies. SETTING: University research laboratory. PATIENT(S): Endometrioma stromal cells (EoSC) were obtained from 28 patients with ovarian endometrioma undergoing laparoscopy. INTERVENTION(S): EoSC were cultured with inflammatory stimuli or recombinant activin-A, followed by RNA extraction. MAIN OUTCOME MEASURE(S): Activin mRNA expression was evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR), and activin-A concentration of supernatant of cultured EoSC was evaluated by ELISA. Also, the effect of activin-A on EoSC was evaluated with real-time RT-PCR and cell proliferation assay. RESULT(S): Inflammatory stimuli, interleukin (IL) -1beta, and tumor necrosis factor (TNF) -alpha induced inhibin/activin-betaA subunit mRNA and activin-A protein expression in EoSC. Additionally, activin-A enhanced EoSC proliferation and increased the expression of IL-6 and protease-activated receptor (PAR)-2 mRNA. CONCLUSION(S): An in vitro study revealed that activin-A, which is induced by IL-1beta or TNF-alpha, might promote endometriosis by stimulating IL-6 and PAR-2 mRNA expression and increasing the proliferation of EoSC. Mesh Terms: Activins/*biosynthesis/physiology| Cell Proliferation/drug effects| Cells, Cultured| Endometriosis/*metabolism/pathology| Female| Gene Expression Regulation| Humans| Interleukin-1beta/*pharmacology| Interleukin-6/*biosynthesis/genetics| RNA, Mess |
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